Present your answers in complete sentences No need to rewrit

Present your answers in complete sentences. No need to rewrite the questions. Instead, to answer question 1, you can write, “In the virtual lab experiment, DNA microarrays were used to bla..bla…”.

Part II. Go through the DNA Microarray Virtual Lab at:

http://learn.genetics.utah.edu/content/labs/microarray/

Explore the page. Click on the microarray to begin. For most of the questions below, click on “Chapter 3: The experiment”.

1.A DNA microarray is used to quantify mRNA levels. Why is it called DNA microarray then, instead of RNA microarray?

2.What is the advantage of DNA microarrays over other methods to study gene transcription?

3.In this particular experiment, what was the objective of the DNA microarray experiment?

4.In part 3 (isolate mRNA), how were mRNAs separated from tRNAs and rRNAs?   What were on the beads?   What unique property of mRNAs enabled its separation from tRNAs and rRNAs?

5.In part 4 (make labeled DNA copy), briefly describe how labeled cDNAs were generated. Practice using technical terms that you’ve encountered in your professor’s highly informative lectures. Describe the primers and enzymes that were used. Explain the reason for the choice of primer/s. Explain what the “special enzyme” does.

6.Parts 5 and 6. What do the colors red, yellow, and green represent? What do the dark spots mean?

7.Part 7. If you’re a researcher developing a drug to shut off genes that are expressed mostly in cancer cells, will you try to turn off genes that showed up green, red, or yellow?

8.Give two reasons or examples as to why DNA microarray experiments don’t always identify all genes that have “gone bad”. Answers are mentioned in the interactive.

Solution

Ques- 1.A DNA microarray is used to quantify mRNA levels. Why is it called DNA microarray then, instead of RNA microarray?

DNA microarray is useful to specifically detect mRNA levels or expression of various genes simultaneous can be meticulously monitored using specific probes when it is transcribed. SO that RNA microarray cannot be used in this context because DNA microanalysis used to monitor genotype multiple regions of a genome. High throughput DNA and protein expression profiling are potential techniques to denote genomic variations associated with mRNA expression, protein modification. These techniques predominantly have useful in identifying DNA sequences through DNA microarray assays.

Answer:

QUes-2.What is the advantage of DNA microarrays over other methods to study gene transcription?

DNA microarray analysis of gene and their mRNA components followed by complementary DNA synthesis is useful to unravel the genome of bacteria. DNA recombination techniques are also useful to unravel the mechanism of genes involved in the microbe

DNA microarray technique is used to detect the SNP of suspects with the DNA samples that were collected from crime scene. If the SNPs of any Suspect and Crime scene SNPs are matched, then he will be considered as an accused.