The two sequences below have been aligned Calculate to score

The two sequences below have been aligned. Calculate to score for this pairwise alignment, given a match score of 0, a mismatch score of 1, a gap cost of 5, and a gap-extension cost of 1
TAACCAGCTTTTCT,TTACAGCGGTCTTTCT

-TAACCAGCT---TTTCT
T..--....GGTC.....

19

22

23

27

Solution

Gap cost means(- - -) 5, gap-extension (….) cost= 1

Mismatch score= 1, match score of =0

On matching the sequences as following:

TAACCAGCT TTTCTTTACAGCGGTCTTTCT

- T AACCAGCT---    T TTCTT . .   - - ....GGTC.....

The score must be following the following way:

5+1+0+1+0+1+1+1+1+0+5+5+5+0+0+0+1+1+1+…

Then can calculate scores.