WOULD YOU CONSIDER AN EIA OR WESTERN BLOT AS BEING A MORE SE

WOULD YOU CONSIDER AN EIA OR WESTERN BLOT AS BEING A MORE SENSITIVE ASSAY? ,EXPLAIN YOUR REASONING.

Solution

Enzyme Immuno Assay or Western Blotting assay is considered as most sensitive assay. Becaues these are all directly or indirectly depends and exploits Enzyme catalysis for the recognition of antigen presence. As enzymes are biocatalysts and show high specificity towrds substrate, the result that is obtained by these techniques are more reliable than other techniques like PCR.

Moreover in the analytes used in these techniques are Monoclonal antibodies, which specifically bind to target. And another most importent thing is here antigen concentration determined using proteins, which are end products of gene expression, which gives more reliable information.

Based on above explanation, the EIA or Western Blotting techniques are more sensitive assays