The enzyme PspN4I cleaves the sequence GGNNCC N any of the f

The enzyme PspN4I cleaves the sequence GG/NNCC (N= any of the four bases). How many times would you expect PspN4I to digest a 30 kb genome? Round off to the nearest whole number.

Solution

The probability of PspN4I cleavage site is = As it has six nucleotides in the recognition sequence, 1/4 x 1/4 x1/4 1/4 x1/4 x 1/4 = 1/4096, which means for we can expect the restriction site for every4096 bases.

In 30 kb genome, 7.3 (30000/4096) ~ 7.0 restriction sites would expect.