6 Question 516 Flies from a Dichaete stock are crossed to ho

#6 Question 5.16 Flies from a Dichaete stock are crossed to homozygous ebony, pink flies, and the F1 progeny, with a Dichaete phenotype, were backcrossed to the ebony, pink homozygotes.

Phenotype Number

Dichaete 401

ebony, pink 389

Dichaete, ebony 84

pink 96

dichaete, pink 2

ebony 3

Dichaete, ebony, pink 12

wild type 13

Identify which categories are noncrossovers (NCO), single crossovers (SCO), and double crossovers (DCO) in each case. Then, indicate the relative frequency in which each will be produced.

Solution

P=   D + +/ + + + (Dicahete)    X     + e p/ + e p (homozygous ebony, pink)

F1= D + +/ + e p (Dicahete)     X     + e p/ + e p(homozygous ebony, pink)

F2 progeny

Phenotype

Genotype

Number

Type of crossover

Dichaete

D + +/ + e p

401

Parental combination or non-recombinants

Ebony, pink

+ e p/ + e p

389

Parental combination or non-recombinants

Dicahete, ebony

D e +/ + e p

84

Single crossover

Pink

+ + p/ + e p

96

Single crossover

Dichaete, pink

D + p/ + e p

2

Double crossover

Ebony

+ e +/ + e p

3

Double crossover

Dichaete, ebony, pink

D e p/+ e P

12

Single crossover

Wild type

+++/ + e p

13

Single crossover

Crossover frequency between D and p= Number of recombinant progeny/total progenyx100= (12+13+2+3/1000)x100=3 map units

Crossover frequency between p and e= Number of recombinant progeny/total progenyx100= (84+96+2+3/1000)x100=18.5 map units

Based on the results obtained in double crossover we can say that the genotypes of parents in F1 cross is

F1: D + +/ + p e   X   + p e/ + p e

Phenotype

Genotype

Number

Type of crossover

Dichaete

D + +/ + e p

401

Parental combination or non-recombinants

Ebony, pink

+ e p/ + e p

389

Parental combination or non-recombinants

Dicahete, ebony

D e +/ + e p

84

Single crossover

Pink

+ + p/ + e p

96

Single crossover

Dichaete, pink

D + p/ + e p

2

Double crossover

Ebony

+ e +/ + e p

3

Double crossover

Dichaete, ebony, pink

D e p/+ e P

12

Single crossover

Wild type

+++/ + e p

13

Single crossover

#6 Question 5.16 Flies from a Dichaete stock are crossed to homozygous ebony, pink flies, and the F1 progeny, with a Dichaete phenotype, were backcrossed to the
#6 Question 5.16 Flies from a Dichaete stock are crossed to homozygous ebony, pink flies, and the F1 progeny, with a Dichaete phenotype, were backcrossed to the

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