Choose the accurate answer To view live cells in a dash we n

Choose the accurate answer To view live cells in a dash we need a/in inverted microscope up-right microscope electron microscope good microscope Blood serum must be added to mammals an cell media because, it has nutrient components it has growth factors & signaling molecules that include cell growth. Mammalian cells need blood Blood serum is required for oxygen &CO;_2 transport mammalian cells grows until they cover the entire surface of the first of the duty/flask where they touch on another point they stop growing this phenomenon is correctly known as Overgrowth inheritance touching inhibition contact inhibition growth factor-induced inhibition. Choose the best accurate a when cells crowded on the dish with no or very little spaces between them, we are them as being crowded saturated overgrown confluent when cells stop growing as described in questions 19 & 20 above, to prevent excessive cell death they must be _____ discarded in biohazard container Fed with more rich media to enhance robust growth Provided with growth factors Passaged choose the most accurate answer: To split mammalian first we aspirate the media, cells, then we wash with 1X PBS and apply trypsin wash with 1X PBS and apply media Wash with buffer and apply trypsin wash with 1X PBS and apply pepsin

Solution

1. Upright microscope

2. Blood serum has growth factors and signalling molecules which induce cell growth.

3. Contact inhibition

4. Discard in a biohazard manner.

5. Wash with 1x PBS and then add trypsin.

These are tge methods of animql cell culture and their subculturing is done by passaging.