Wat is the final concentration of wach of the reagents Tris

Wat is the final concentration of wach of the reagents (Tris, Borate and EDTA) in 1xTBE when the stock solution is diluted to 1L? show you work. Thanks in advance.

Solution

Answer :

Let’s calculate the concentration for Tris

108 g Tris in 1000 mL

Given

MW= 121.14

Weight = 108g

Molarity = ?

grams=(molarity x mol wt x ml of solution)/1000

Molarity = (grams x 1000)/ (mol wt x volume of solution)

=(108 x 1000)/121.14 x 1000

=0.891 M

=89 mM

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similarly, Concentrations of other components includes :

89 mM boric acid and 2 mM EDTA