You begin to sequence the genome of Tamatoa gathering 5000 r

You begin to sequence the genome of Tamatoa, gathering 5,000 reads that were each 600 base pairs long. You have hypothesized that the Tamatoa genome is about 2 million bases long.

(a) At what coverage have you sequenced the genome thusfar?

(b) If the coverage of the Tamatoa genome were 6X, what is the probability that a base will be unsequenced?

Solution

Genome coverage = LN/G

where L= average read length

N= no. of reads

G = haploid genome length

Therefore the coverage= 600 x5000 / 2000000

Coverage = 1.5x

b) If the coverage of the Tamatoa genome were 6X, the probability that a base will be unsequenced is zero.