you extract DNA from a sample and estimate it to be at a con

Solution

Explanation: The following is procedure which is used to extract DNA.

Agarose gel electrophorosis is the technique which is used to seperate DNA fragments based onthere molecular weight.

This technique consists of 3 basic steps

1)preparation of agarose gel

2)electrophorosis of DNA fragments

3)visualizing

Preparation of agarose gel:

Agarose is a linear polymer extracted from sea weeds,which is soluble on boiling.molten agar solution is poured into a mould and allowed to solidify.As it cools agarose undergoes polymerization and was converted to gel.Pore size and densiity of the gel is depended on concentration of agarose init.

Preparation of 1% agarose gel:

1) Prepare 1*TAE buffer by diluting the amount of 50*TAE.

2)weight 1 gram of agarose and add to 100ml of 1*TAE.This gives 1% agarose gel.

3)Boil till agarose dissolve completely.

4)mean while place the combs on electrophorosis set

5)pour the agarose solution in central part of the tank.the thickness of the gel should be 0.5-0.9cm ,and leave the gel undisturbed for agarose to get solidify.Ethidium bromide is a staining material used to track the DNA fragments,this can be added to the gel or in to buffer( add 1 g of dye to 100ml distilled water) and add 0.5ul of staining solution to the solidified gel.

6)pour 1*TAE buffer into the gel tank till buffer level stands 0.5cm above the gel.

7)gently lift the combs ensuring that the wells remain intact.

8)connect to the power supply ,where red:anode,black:cathode.

9)load the sample 20 ul of a 50 ng/ul in to the wells.

10) adjust the voltage to 50,

11)Switch of the power when tracking dye reaches 3/4 of the gel. And this whole prcedure takes 1 hour of time.

By this agarose gel electrophorosis technique DNA fragments based on the molecular weight will be seperated.