Give a detailed introduction of Dark Field Microscopy and it

Give a detailed introduction of Dark Field Microscopy and it\'s significance to cell biology.

Solution

Dark-field microscopy is a very simple and effective technique and is used to view live and unstained biological samples. On the specimen, the hollow cone of light is focused in such a way, that the unrefracted and unreflected rays will not enter the objective. So, light which was actually refracted or reflected by the specimen will form the image. Here the field surrounding the specimen will look dark and object will look illuminated. Due to this, we can see the internal structures present in the cell.

It is very important in cell biology as it allows you see the cells more clearly and detail of variety of specimens. Using this one can yield high magnification in case, of bacteria and can view tissues and cells with low magnification. Using this, scientists have studied a number of bacteria and fungi in detail.