1 what is a recombinant protein and why would it be used ins

1. what is a recombinant protein, and why would it be used instead of a a native protein? 2. Name one pro and one con of using eukaryotic cells to produce a recombinant prote When would you choose to use eukaryotic cells to produce a recombinant protein? 3. Name at least one pro and one con of using prokaryotic cells to produce a recombinant protein. When would you choose to use prokaryotic cells to produce a recombinant protein? 4. What are three considerations for recombinant protein gene design? Hint: think about the eventual goals. 5. What is the log phase of a cell culture, and why is it important to recombinant protein production?

Solution

1. a). Recombinant protein is a protein those code is carried by a recombinant DNA. Recombinant DNA means segment of DNA in a plasmid. Once rDNA is inserted in bacteria, it will make protein based on this rDNA.

b) It is used to get human growth hormone, insulin, vaccines etc. As they can be produced by recombinant bacteria. These recombinant proteins can be used in medical application.

2. In eukaryotic cells yielding is slower and usually higher production. But some times you can encounter hyperglycosylation

3. a) Bacteria can express large amounts of recombinant protein, but proteins sometimes do not fold properly. Folding of protein is essential for proper function of protein.

b) Bacteria can produce recombinant protein in less time and cheap. Bacterial cell cannot carry out post-translation modifications

4. There three considerations for recombinant protein design are 1) Replicon, promoter and selection marker, and also affinity tag, removal tags

5. Log phase of bacterial cultural is period characterized by cell doubling. In this phase bacterial growth is exponential. As reproduction and growth of bacteria is more the production of recombinant protein is also more.