you feel the rate of the reaction is no longer affected by a

you feel the rate of the reaction is no longer affected by adding a higher concentration and amylase. wa 6 drops distilled of lase and distilled water, 4 drops amylase 9. Graph your results on the Enzyme Studies Worksheet. Then write a statement about the relationship betwe tration of amylase and its ability to digest starch. ative Part III. pH l. Using a clean reaction plate, add 2 drops of starch solution to cach of three successive wells 2. To well l add 1 drop of 0.1 M hydrochloric acid solution. To well #2 add l drop of distilled water. To well #3 add 1 drop of 0.1 M sodium hydroxide solution. 3. Add 8 drops of distilled water to each of the three wells. 4. Use small strips of pH paper to determine the pH of the solution in each well. 5. Add 2 drops of amylase solution to each well. 6. Wait three minutes and add a drop of iodine to all three wells 7. Record the results on Part lll of the Enzyme Studies Worksheet. 8. Answer the question for Part lll on the worksheet. X Part Temperature 1. Using a clean reaction plate, add 2 drops of starch solution to each of three successive wells. n ,,,alu add Q drons f boiling water

Solution

This experiment shows the activity of amylase enzyme at different pH. amylase is an enzyme which act on startch and degrade it. Iodine in the presence of starch shows dark brown colur. The well 1 having pH 2 and dark brown colour shows the presence of startch in the well (since iodine produce colour in the presence of startch). The well 2 having pH 6 and having dark brown colour also show the presence of startch in the well. Hence the amylase present in these two well (1 & 2) did not show any activity against startch. In the well 3 having pH 9 and disappearance of iodine colour shows the absence of startch (since iodine does not produce colour in absence of startch. It means amylase present in well 3 has shown the activity and degraded the startch. Hence by this experiment it can be concluded that the activity of amylase enzyme is less at low pH value (pH = 2, 6) and high at higher pH value (pH = 9). The graph will be parabola.