After 30cycle PCR amplification please calculate the percent

After 30-cycle PCR amplification, please calculate the percentage of each type of PCR product at the end the reaction

3RD cycle NO NO 30TH cycle Predominant product because of exponential increase in copy number

Solution

Number of copies of DNA obtained after \'n\' cycles= 2(n+1)

=2(30+1)=231= 63billion copies.

Percentage: 63billion /30 *100

After 30-cycle PCR amplification, please calculate the percentage of each type of PCR product at the end the reaction 3RD cycle NO NO 30TH cycle Predominant pro

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