In addition to the extracts and the plasmid DNA are there an

In addition to the extracts and the plasmid DNA, are there any additional materials you should add to this in vitro replication system? Explain your answer Which of the following statements is true with respect to this in vitro replication system? There will be only one leading strand and one lagging strand produced using this template. The leading and lagging strands compose one half of each newly synthesized DNA stand. The DNA replication machinery can assemble at multiple places on this plasmid. One daughter DNA molecule will be slightly shorter than the other. You decide to use different bacterial strains (each having one protein of the replication machinery mutated in order to examine the role of individual proteins in the normal process of DNA replication. What part of the DNA replication process would be most directly affected if a strain of bacteria lacking primase used to make the cell extracts? initiation of DNA synthesis Okazaki fragment synthesis leading-strand elongation lagging-strand completion

Solution

11.Taq polymerase, a heat resistant DNA polymerase, so that it can remain intact during the DNA denaturation process.
Two primers
Deoxynucleoside triphosphates (dNTPs, sometimes called \"deoxynucleotide triphosphates\"; nucleotides containing triphosphate groups), building-blocks of the DNA polymerase synthesizes a new DNA strand.
Buffer medium, providing a suitable chemical environment for optimum activity and stability of the DNA polymerase
Bivalent cations, magnesium or manganese ions; generally Mg2+ is used, but Mn2+ can be used for PCR-mediated DNA mutagenesis, as higher Mn2+ concentration increases the error rate during DNA synthesis.
Monovalent cation potassium ions
are needed for invitro DNA replication.

12. The answer is A. There will be only one leading strand aand one lagging strand producced using this template.

13. 13. Initiation of DNA syntheis. Primer, The Starter, is a product of Primase