You will be asked to make 20 ml of 10X loading dye Using the

You will be asked to make 20 ml of 10X loading dye. Using the information above, provide a recipe for 20 ml of 10X loading dye. How much of each of the two dyes will you weigh out? How much of the glycerol solution that you made in question #2 will you be using?

Solution

Recipe for gel loading dye 10X

7.8 mL glycerol

1.0 mL 10% w/v SDS

400 uL 0.5M EDTA

0.05g bromophenol blue

0.05g xylene cyanol

Bring the volume to 20 mL with water.