how would you do the following Prepare 15mL of TE buffer 10m

how would you do the following: Prepare 15mL of TE buffer (10mM Tris and 1mM EDTA) from sterile stocks of 1M Tris and 0.5M EDTA and DI water?

Solution

Answer:

Preparation of Tris

Stock= 1M Tris

Required = 10mM=0.01M

Volume = 15 mL

C1V1= C2V2

(stock)   (required)

V1=C2V2/C1

V1 = ( 0.01M X 15 mL )/ 1M

=0.15 mL

Preparation of EDTA

Stock= 0.5 M EDTA

Required = 1mM=0.001M

Volume = 15 mL

C1V1= C2V2

(stock)   (required)

V1=C2V2/C1

V1 = ( 0.001M X 15 mL )/ 0.5M

=0.03 mL

Preparation of Tris-EDTA (TE buffer)

0.15 mL of Tris from stock + 0.03 mL of EDTA from stock of ETDA + 14.82 mL of H2O