During immunistaining process Note that you will incubate ov

Note that you will incubate overnight (2 nights actually) and come back on Wednesday to finish. Stop and Think: What types of issues might arise when incubating antibodies onto tissues for longer than usual? Why? Assuming the potential problems, you raised in the previous question do arise, what are your strategies to overcome them?

Solution

The incubation time is given to the certain reactions so that the particular antibodies can binds to the specific antigens etc.. When the incubation time exceeds the given time it can leads to non-specific binding too which can result in the change in the final result or can give wrong interpretation..!! \'if the temperature pressure also changes during this date but ek baar...

The stragies are in to perform the reactions in the proper incubation time.. With proper condition like appropriate concentration. Use a blocking buffer so that the unwanted places can be easily blocked to prevent the binding of antibodies to the.