Does DNA react with the biuret reagent Does DNA enhance inhi

Does DNA react with the biuret reagent?

Does DNA enhance, inhibit, or not affect the reaction of the biuret reagent with the peptide bonds?

Solution

Biuret reagent is composed of an alkaline mixture of potassium hydroxide, copper sulphate and potassium sodium tartrate. copper present in the copper sulphate reduces from +2 to +1 upon reaction with the peptide bond and change its colour. Since DNA has phosphodiester bond and not peptide bond so DNA does not react with biuret reagent. DNA does not enhance, inhibit, or not affect the reaction of the biuret reagent with the peptide bonds.