a Using restriction enzyme digests and gel electrophoresis d

a. Using restriction enzyme digests and gel electrophoresis, describe the number and size of fragments (bands on a gel) you would expect for the ligation product above after digestion with each restriction enzyme(be specific).

Solution

In the ligation product, there are 3 restriction sites, 2 of EcoRI and 1 PstI. This means that those 3 sites are where we can cleave the product at. If we were to use restriction enzymes for both EcoRI and PstI and gel electrophoresis; digestion will occur at the 3 indicated sites- 2 for EcoRI and 1 for PstI and we will get;