Explain 3 nanomoles of the free amino acid tyrosine gives le

Explain: 3 nanomoles of the free amino acid tyrosine gives less reaction in the Lowry assay than 1 nanomole of a tripeptide containing 3 tyrosines.

Solution

The no of molecules are more in 1 mole of tyrosine tripeptide in compare to 3 nanomole of free amino acid that that means it have more number of hydroxyl phenol group that reduce the phospho molybdate present in the F/C reagent to form Blue color.