What are some consequence of leaving on a bacterial smear to

What are some consequence of leaving on a bacterial smear too long (over-staining) ?: What are some consequences of not leaving a stain on a smear long enough.

Solution

Rod-shaped bacteria (Baccillus)

Average diameter = 1.5.µm

Therefore, average radius = 1.5/2 µm = 0.75 µm

Average length = 4 µm

Surface area = 22.38 µm² (approximately)

Volume = 7.07 µm³ (approximately)

Spherical bacteria (Coccus)

Average diameter = 2 µm

Therefore, average radius = 2/2 µm = 1 µm

Surface area = 12.57 µm² (approximately)

Volume = 4.19 µm³ (approximately)

Organism	Cell morphology	Surface area (µm2)	Volume (µm3)	Surface-to-volume-ratio
B. cereus	Rod shape	22.38	7.07	3.17
M. luteus	Coccus	12.57	4.19	3.0