The following tiles indicate the first five thermocycler ste

The following tiles indicate the first five thermocycler steps in a PCR run. Arrange them in the correct order. Rank from first to last. Do not overlap any tiles.

72C for 1 minute

55C for 1 minute

94C for 1 minute

94C for 1 minute

55C for 1 minute

Solution

1. PCR involves process of heating and cooling called thermo cycling. There are three states, they are 1. Denaturing 2. Annealing and 3. Elongation.

2. In these three stages different temperature is being maintained. The order of temperature is as follows.

1. 94^oC for 1 minute; At this temperature DNA is denatured.

2. 55^oC for 1 minute: At this temperature anneals primer with single stranded DNA.

3. 72^oC for 1 minute: This is optimal temperature to synthesize new DNA strand.

4. 94^oC for 1 minute; At this temperature again complementary and template DNA is denatured.

5. 55C for 1 minute: At this temperature again anneals primer with both complementary and template DNA.to synthesize new DNA strand.