What are the advantages of bacterial structures to the Gram

Solution

Gram stain is specifically used to detect the gram positive & gram- negative bacterial species with a clear identification comapted to methylene blue staining. For example, Gram negative E. coli staining is going to stain its cell wall that is consist of a thin peptidoglycan layer associated with outer membrane finally this cell wall wall layer is useful to for \"bacterial structures\" to the Gram staining technique for classification of bacteria. Gram-positive bacteria cells possess a thick mesh like peptidoglycan layers. Gram-negative bacteria possess specific cell wall structure and they have three main components, which enable to resist entry of high molecular weight antibiotics (penicillins) as these bacteria possess a complex “envelope”, “layer of peptidoglycan” and a “periplasmic space” & specific lipopolysaccharide

For example, leprosy caused by mycobacterium leprae (a type of bacteria). These bacteria identified by gram staining. Gram-positive bacteria give the positive result with the Gram staining and gram-negative bacteria give the negative result. Staphylococcus aureus and mycobacterium leprae are gram-positive bacteria and give the positive result with gram staining.

Escherichia coli (E. coli), a Gram negative and a rod-shaped member of the coliform group bacteria, can be this is an \"enteric bacteria\"

Gram staining: This is a bacterial staining technique was investigated by scientist Christian Gram. It is used to identify and classify the bacteria based on the cell wall fluorescence. Normally bacterial \"peptidoglycan cell wall structure\" is negative and stained with crystal violet dye (primary stain) and if it retains the dye in the presence of iodide (mordant which fixes the dye to the cells). Then it is said to be Gram (+) positive and if it does retain the pink colour after adding a counter stain (safranin) then it said to be Gram (-) negative. Iodide used to fix crystal violet dye by reacting with the dye (Iodide (Ior I3) interacts with CV+) inside the heat fixed bacterial smear therefore, dye can be easily loaded into the dead bacterial cells to visualize under the microscope. If the omission of iodide has done and leading to inability of crystal violet dye to trap it in the bacterial cell therefore, staining cannot be achieved.

Safranin is the counter stain used. After alcohol or acetone washings of heat fixed bacterial smears, It is necessary to retain the pink colour after adding a counter stain (safranin) then it said to be Gram (-) negative. Other than safranin, Carbol fuchsin is sometimes used to identify anaerobic bacteria.

Gram-staining of particular bacterial specimens has meticulous advantages \"based on bacterial cell wall structures\" & endospore scans obtained from biopsies of infected tissues from any organism indentifying a particular disease. It is essential to use specific dye to unravel the type of organism that caused any particular disease or disorder. Therefore, it is easier to determine the exact treatment protocol against that infection. It is difficult to study the exact microbiological properties without proper microbial staining techniques and existence is a bit doubtful because staining (Gram staining) of the microbial specimens using specific dyes (crystal violet, methylene blue, safranin) determines the microbial morphology of the organism to identify a specific antibiotic against any disease.