In affinity chromatography the solid support is coated with

In affinity chromatography, the solid support is coated with a ligand to which the protein of interest binds tightly but reversibly. To elute the protein from the column, one uses a solution containing a chemical that is__the solid support ligand. identical or similar to very different from Which of the following is generally NOT used in the large-scale purification of a protein? Salting out ton-exchange chromatography Size-exclusion chromatography Affinity chromatography Electrophoresis

Solution

3) Chemical agent should be different to elute protein. The solvent molecules elute or travel down through the chromatography column they can either pass by the adsorbent or analyte complex or they can displace the analyte by binding to the adsorbent in its place. After the solvent molecules displace the analyte the analyte can be carried out of the column for analysis.

4.) Ligands used in affinity chromatography are expensive and leakage of ligands is observed sometimes and productivity is low with affinity chromatography.