In the lab you are presented with a yellowish unknown liquid

In the lab, you are presented with a yellowish, unknown liquid. You want to determine whether or not it is 1) polar, 2) a lipid, 3) saturated or unsaturated, and 4) mixed or unmixed. What is the most logical order in which to complete the following steps, what results do you expect from each step and what conclusions can you draw from each step?

Arrange the followig steps in order 1-12

___perform the paper test

  

____the substance does not mix with water

___the substance is a liquid

___observe if the substance is a liquid or solid

___mix with water

  

___the substance leaves three spots on the TLC strip

___the substance is likely an unsaturated lipid

____perform thin layer chromatography

  

____he substance leaves a transparent spot

____the substance it is likely a lipid

_____the substance is non-polar and may be a lipid

_____the substance is likely a mixed lipid

Solution

___observe if the substance is a liquid or solid ___the substance is a liquid ___mix with water ____the substance does not mix with water..........mostly fats do not mix with water. Fats are lipids which have non-polar covalent bonds. Fats are hydrophobic and do not dissolve in water. so, fats could be said to dislike water. _____the substance is non-polar and may be a lipid........fats are saturated or unsaturated. If saturated, the fats are solid at room temperature and if unsaturated, the fats are liquid at room temperature.    ___the substance is likely an unsaturated lipid........because the given sample is liquid at room temperature.    ___perform the paper test...........paper chromatography helps in separating amino acids. Paper chromatography is also a type of thin layer chromatography. The substance within the mixture gets separated according to their polarity. ____the substance leaves a transparent spot ____the substance it is likely a lipid ____perform thin layer chromatography.....which helps in separating non-volatile mixtures. ___the substance leaves three spots on the TLC strip.........shows presence of three substances in the mixture.    _____the substance is likely a mixed lipid