ower or else the objective lens will break the microscope sl

ower, or else the objective lens will break the microscope slide. The objective lens itself can be easily damaged by such an accident. Numerical aperture (related to Working distance Magnification resolution) 5.1 mm 0.25 0.15 mm 0.65 90 (immersion) 0.05 mm 1.25 The resolving power of a microscope may be calculated using the Abbey equation: ds 0.612% NA d resolution (nm) wavelength of light used (yellow-green light 5200-5800 A) NA numerical aperture

Solution

Magnification 10x

Resolution = (0.612 x Lambda)/Numerical aperture

Given

Lambda = Visible light has an average wavelength of 0.55 um

NA= 0.25

Resolution = (0.612 x 0.55)/0.25 =1.34 micrometer

Magnification 40x

Resolution = (0.612 x 0.55)/0.65 =0.51 micrometer

Magnification 90x

Resolution = (0.612 x 0.55)/1.25 =0.26 micrometer