On a given microscope the numerical apertures of the condens

On a given microscope, the numerical apertures of the condenser and low-power objective lenses are 1.25 and 0.25, respectively. You are supplied with a filter that selects a wavelength of 520 nm. What is the limit of resolution on this microscope? Will you be able to distinguish two points that are 300 nm apart as being separate, or will they blur into one?

Solution

4). The light waves have a characteristic property, i.e. the wave length. The distance between two successive peaks is known as “wave length.” Amplitude is the height of each peak. The waves with the higher frequency has the highest energy. And the waves with the lower frequency has the lowest energy.

Resolution mainly depends on wavelength in light microscope because the higher frequency waves (low wavelength) are better shown by the ocular lens. This increases the resolution.