Homework SoursePlace the following steps for chain termination of bacterial
Place the following steps for chain termination of bacterial protein biosynthesis in the correct order:
1) Release factor 1 binds to the mRNA stop codon.
2) Release factors 1 and 2 as well as GDP dissociate from the complex, followed by the release of the tRNA.
3) The UGA (or UAA or UAG) stop codon aligns with the A site.
4) The 50S subunit dissociates.
5) Release factor 1 binds to the mRNA stop codon.
6) Peptidyltransferase catalyzes the hydrolysis of the peptidyl-tRNA bond to release the newly synthesized peptide.
7) The completed peptidyl-tRNA is in the P site.
Place the following steps for chain termination of bacterial protein biosynthesis in the correct order:
1) Release factor 1 binds to the mRNA stop codon.
2) Release factors 1 and 2 as well as GDP dissociate from the complex, followed by the release of the tRNA.
3) The UGA (or UAA or UAG) stop codon aligns with the A site.
4) The 50S subunit dissociates.
5) Release factor 1 binds to the mRNA stop codon.
6) Peptidyltransferase catalyzes the hydrolysis of the peptidyl-tRNA bond to release the newly synthesized peptide.
7) The completed peptidyl-tRNA is in the P site.
| 7, 2, 5, 3, 6, 1, 4 |
| 7, 6, 3, 1, 5, 2, 4 |
| 7, 3, 5, 1, 6, 2, 4 |
| 7, 5, 2, 3, 6, 1, 4 |
Solution
Answer:7, 3, 5, 1, 6, 2, 4
7) The completed peptidyl-tRNA is in the P site.
3) The UGA (or UAA or UAG) stop codon aligns with the A site.
5) Release factor 1 binds to the mRNA stop codon.
4) The 50S subunit dissociates.
1) Release factor 1 binds to the mRNA stop codon.
6) Peptidyltransferase catalyzes the hydrolysis of the peptidyl-tRNA bond to release the newly synthesized peptide.
2) Release factors 1 and 2 as well as GDP dissociate from the complex, followed by the release of the tRNA.
