An investigator attempting to replicate Pasteurs swannecked

An investigator attempting to replicate Pasteur\'s swan-necked flask experiment has boiled a hay infusion for 1 hour. However, she observes growth in the flask after 1 day (even when undisturbed). What is the likely explanation for this result?

Solution

Hey infusion is a mixture which is made by taking ponds, lakes, rivers water etc. It is one of the best method to increase the bacterial cell density at given time..!!

It can be due to the certain reasons that the hay infusion may have contain some endospore which is resistant to heat or may contain some types of bacteria that can tolerate high temperature..

Due to which even after heating for 1 hours it did not get affected by the heat and gets the chance for growth after the heat is off and when the sample is left afterwards...!!