The baking yeast is the preferred organism to generate biofu

The baking yeast is the preferred organism to generate biofuels. However, it requires processed sugar to generate ethanol, which may put a strain on the food industry. Therefore, you decide to choose a photosynthetic organism to generate bioethanol. You select cyanobacteria. which use the sunlight as the primary energy for cell growth and proliferation. What are the two enzymes for which you would like to clone the coding genes and express in cyanobacteria? What are their respective substrate(s) and product(s)? Name the enzyme(s) that is/are also expressed in humans and describe its/their functions.

Solution

ans. The roke of yeast in production of ethanol is the most important element that distinguishes wine from grape juice,

In the absence of oxygen that is under anerobic conditions yeast converts the sugar into ethanol and carbondioxide throufg the process of fermentation.

1, The two enzymes are pyruvate decaroxylase(pdc) and alcohol dehydrogenase. (adh)

The coding sequence from both the enzymes frm the bacterium zymomonas is cloned into a shuttle vector pCB4r and then transform into cynobacteria strain

2. The major pathway for synthesis of bioethanol involves

PDC catalyses the non oxidative decarboxylation of pyruvate which results in the production of acetaldehyde and carbon dioxide

Acetaldehyde is then reduced to alcohol by alcohol dehydrogenase.

PDC is tetramer of 4 identical subunit of 50kd mass.

2 isozymes of ADH present.

3. Enzymes in human for ethanol production is

ADH4 which is present in gut