Homework Soursehen we amplify a gene by PCR and before we can introduce it
hen we amplify a gene by PCR, and before we can introduce it into yeast or bacteria, it has to be:
A)Inserted into a linear DNA vector that becomes circular after the gene has been inserted.
B)Sterilized, or else whatever organism we introduce it into can become contaminated.
C)Mixed with a DNA vector that produces Ampicillin.
D)Mixed with carefully designed primers that will help it get expressed correctly.
E)Inserted into a lipid vesicle that will fuse with the cell and deliver the gene into the bacteria or yeast.
| A)Inserted into a linear DNA vector that becomes circular after the gene has been inserted. | ||
| B)Sterilized, or else whatever organism we introduce it into can become contaminated. | ||
| C)Mixed with a DNA vector that produces Ampicillin. | ||
| D)Mixed with carefully designed primers that will help it get expressed correctly. | ||
| E)Inserted into a lipid vesicle that will fuse with the cell and deliver the gene into the bacteria or yeast. |
Solution
Option A is the correct answer. As in recombinant DNA technology a gene of interest is amplified using PCR. After that it is inserted in the plasmid specific for the particular host be it bacteria or yeast. After ligation is done the product is known as Recombinant molecule which is then transformed into the organism.
