5 What is the purpose for making a smear during negative sta

5. What is the purpose for making a smear during negative staining?

Solution

Answer:

Negative staining & smear preparation: Smear preparation during negative staining is very essential to view “bacterial or microbial strains” without distorting their structure or without using heat fixation thereby; it is possible to view bacterial motility or bacterial conjugation. Harsh dyes need not be applied to the smear so that it is possible to view bacterial motility easily

Negative stain: Normally bacterial cell wall is negative and stained by a dye called nigrosin. In this aspect, a proton taken from the dye chromophore followed by no considerable staining cell wall but staining observed on endospores (ex. Bacillus endospores).