can RNAseq be used instead of Reverse transcriptase PCR to q

can RNA-seq be used instead of Reverse transcriptase PCR to quantitatively measure the CTGF mRNA expression

Solution

Yes it can be used to measure the mRNA level and it will give more accurate quantification but it is more specific tecnique used to analyze the continually changing cellular transcriptome. to compare the mRNA level of two different sample like drug treated and untreated. For normal purpose quantification qith RT-PCR is best option for some specific purpose where you need more accuracy you cna prform chip-Seq.