Fluorescent dyes can be attached to large fragments of DNA b

Fluorescent dyes can be attached to large fragments of DNA by any of these methods except ______.

A. attaching the dye to oligonucleotides and then using the oligonucleotides for DNA synthesis (by PCR)

B. labeling the bases of the nucleotides with the dye and using the nucleotides to synthesize DNA

C. inserting the free dyes into a cell and having the cell incorporate the dye in its DNA

Solution

Answer : The correct answer is C ( inserting the free dyes into a cell and having the cell incorporate the dye in its DNA). This method cannot be used to label DNA molecules as free dyes cannot be incorporated into the DNA strands.