BamHI will cut pUC19 and pRecA plasmid DNA how many timesSol

BamHI will cut pUC19 and pRecA plasmid DNA how many times?

Solution

The BamH1 have six bases in recognition site hence the probable restriction site is 1/4 X 1/4 X 1/4 X 1/4 X 1/4 X 1/4 = 1/4096, which means there is a change of BamH1 recognition site for every 4096 base pairs.

pUC19 has 2686bps. The BamH1 site is included in MCS. along the that the proportion is very less.

The occurance of BamH1 site is even less in case of pRecA because of the 3.2 size. The proportion of recognisition site is very less. It is better to use the restriction enzymes whcih have five (1/1024 is the probability of recognition site) or four nitrogen bases (1/256 is the probability of recognition site) in the recognition site.

If you take pUC19, it has the BamH1 site in MCS. Please do verify this.

Plesae let me know the plasmid length and image of pRecA, so I can hlep you.