Answer the following questions below involving the graph bel

Answer the following questions below involving the graph below.

A.) Based on the graph provided and understanding of brush boarder cell biology, in comparison to untreated WT (Wild Type) cells cholera toxin treatment causes ______________________ ( Decreased or Unchanged or Increased) chloride efflux, cftr- mutations cause ______________________ (Decreased or Unchanged or Increased) chloride efflux, PDI treatments cause _________________ (Decreased or Unchanged or Increased) chloride efflux, and DDA treatments cause _____________________ (Decreased or Unchanged or Increased) chloride efflux.

B) The tall unlabeled bar on this graph must represent the chloride efflux data from... (Multiple Choice Options Below)

a) WT cells treated with chloera and DDA

b) cftr- cells treated with DDA

c) cftr- cells treated with PDI

d) WT cells treated with cholera and PDI

C) Fill in the blanks with choice options Higher or Lower or Unchanged

cftr- cells treated with cholera toxin would have _____________ chloride efflux levels when compared to untreated wild type cell efflux. Cells treated with both PDI and DDA would have ______________ chloride efflux levels when compared to untreated wild type cells.

SIngle treatments or mutation efflux Control CT CT PDI PDI Or Or or CFTR- CFTR- DDA DDA combined treatment CT PDI AND AND CFTR- DDA

Solution

a) cholera toxin treatment causes INCREASED ( Decreased or Unchanged or Increased) chloride efflux, cftr- mutations cause DECREASED (Decreased or Unchanged or Increased) chloride efflux, PDI treatments cause UNCHANGED (Decreased or Unchanged or Increased) chloride efflux, and DDA treatments cause INCREASED (Decreased or Unchanged or Increased) chloride efflux.

b) option A. WT cells treated with cholera and DDA.