1Which of the following is NOT correct when considering the

1)Which of the following is NOT correct when considering the initiation of transcription in E. coli?

A) The subunit of RNA polymerase makes strong contacts with the -10 region of promoters.

B) Only the -10 region of the promoter is required.

C) DNA melting near the transcription start site is essential for promoter recognition.

D) The phosphodiester bonds that are formed are not stable until about 10 nucleotides have been incorporated.

E) The RNA polymerase contacts about 50 bp of DNA.

2)In bacteria, mRNA, tRNA and rRNA are all transcribed by the same RNA polymerase.(TRUE/FALSE)

Solution

Answer :- option B (only the - 10 region of the promter is required)

Explanation :-The transcription in prokaryotes require both - 10 box also called pribnow box (TATAAT) and - 35 box TTGACA to initiate transcription where RNA pol binds.

2:- Answer True

Explanation :-In bacteria mRNA, tRNA, and rRNA all are transcribed by the same RNA polymerase. There is only single RNA pol in bacteria. It consists of two aloha subunits two beta subunits, omega and epsilon subunit. Whereas in eukaryotes three diffrerent RNA pol are present.