Which statement about Gateway cloning is not true Gateway cl

Which statement about Gateway® cloning is not true? Gateway® cloning vectors exploit a bacteriophage recombination system. Integrase cuts within attB and attP sites. The ccdB gene produces a toxin within host cells carrying the gene of interest. Excisionase regonizes attL and attR sites to remove the recombined DNAfragment. The cloning into the entry vector is necessary to generate attL sites on the ends of the gene of interest.

Solution

Getaway clonning technology is based on the site-specific recombination system used by phage I to integrate its DNA into E.coli chromosome. Both organisms have specific recombination sites called attP in phage I site and attB in E. coli. The integration process is catalysed by 2 enzymes: phage I encoded protein Int (integrase) and E.coli protein IHF (Integration host factor). The correct answer is option c. The ccdB gene produces a toxin within host cells carrying the gene of interest is untrue for getaway clonning.