The restriction enzyme used to cut the PCR product is HaeIII

The restriction enzyme used to cut the PCR product is HaeIII and its recognition sequence is: GGâCC a) What is the orientation of this recognition sequence? b) Write the recognition sequence as a double strand - what do you notice about it?

Solution

a, The orientation of this recognition sequence is 5\'-GG^CC-3\'.

b . The double tranded orientation of this sequence will be

5\'- GG^CC -3\'

3\'- CC^GG -5\'

This sequence is a palindromic sequence, and it will cut the DNA between second and third base. That ius b etween G and C and thus the blunt ends will be produced.

hence it can be said that this restriction enzyme HAEIII is a blunt end cutter,