Homework Sourse13 For the following lac operon partial diploid genotypes in
13.) For the following lac operon partial diploid genotypes, indicate with a (+) or (- ) whether enzymatic activity would be detected for -Galactosidase and/or Permease when lactose is absent and when lactose is present. Solution is given below could you explain how to get them? Thorough step by step explanation will be appreciated.
A.) I+ P+ Oc Z+ Y- / Is P+ O+ Z- Y+
B.) I- P+ O+ Z+ Y+ / I+ P+ O+ Z+ Y-
C.) I+ P- Oc Z+ Y- / I+ P+ O+ Z- Y+
D.) I- P+ Oc Z+ Y- / I+ P+ O+ Z- Y+
E.) Is P- Oc Z+ Y+ / I+ P+ O+ Z+ Y-
F.) I+ P- O+ Z+ Y- / I- P+ OC Z- Y+
Lactose Absent
Lactose Present
b-Gal
Permease
b-Gal
Permease
A
+
-
+
-
B
-
-
+
+
C
-
-
-
+
D
+
-
+
+
E
-
-
-
-
F
-
+
-
+
| Lactose Absent | Lactose Present | |||
| b-Gal | Permease | b-Gal | Permease | |
| A | + | - | + | - |
| B | - | - | + | + |
| C | - | - | - | + |
| D | + | - | + | + |
| E | - | - | - | - |
| F | - | + | - | + |
Solution
A.) I+ P+ Oc Z+ Y- / Is P+ O+ Z- Y+
B.) I- P+ O+ Z+ Y+ / I+ P+ O+ Z+ Y-
C.) I+ P- Oc Z+ Y- / I+ P+ O+ Z- Y+
D.) I- P+ Oc Z+ Y- / I+ P+ O+ Z- Y+
E.) Is P- Oc Z+ Y+ / I+ P+ O+ Z+ Y-
F.) I+ P- O+ Z+ Y- / I- P+ OC Z- Y+
Lactose Absent
Lactose Present
b-Gal
Permease
b-Gal
Permease
A
+
-
+
-
B
-
-
+
-
C
-
-
-
+
D
+
-
+
+
E
+
+
+
+
F
-
+
+
+
IF THE PROMOTER IS CONSTITUTIVE EVEN SUPER REPRESSOR ALSO WILL NOT WORK. USE THIS LOGIC TO GET THE SOLUTION
IF YOU NEED ANY FURTHER CLARIFICAION COMMENT.
| Lactose Absent | Lactose Present | ||||
| b-Gal | Permease | b-Gal | Permease | ||
| A | + | - | + | - | |
| B | - | - | + | - | |
| C | - | - | - | + | |
| D | + | - | + | + | |
| E | + | + | + | + | |
| F | - | + | + | + | |
