need the last two questions in bold answered please thank yo

need the last two questions in bold answered please. thank you

Record the brand of lactase, number of units of lactase/tablet and the expiration date. Weigh one lactase tablet. Note whether the tablet Is 9000 FCC (Food Chemical Codex) or 3000 FCC units/tablet. Grind a tablet using a mortar and pestle until finely ground. Suspend/dissolve to 1000 units/mL in 0.01 M PO_4 buffer, pH 7.0. Dilute 1:1000 with 0.01 M PO_4 (pH 7.0). The total volume of diluted enzyme should be 10 mL. You will use this diluted enzyme In both parts 2 and 3. Keep on ice.

Solution

Generally, tablets contain 9000 FCC units for their medication use. To convert 9000 units into a solution at 1000units per ml, we use following formula

U₁ x V₁ = U₂ x V₂ where U is units and V is the volume.

Here we take 9000 units of tablet is dissolved in 1 ml of water.

=9000 x 1 = 1000 x V₂

V₂ =9 ml

Therefore, we need 9 ml of water to make 9000 units of tablet into 1000units/ml solution.

We need to make dilution in such a way that it takes final 10 ml solution after diluting in 1:1000 dilutions. For that, we use formula here:

P₂ x V₁ = P₁ x V₂, Where, for the initial volume, we take 1 proportion and 1000 is taken as 2^nd proportion.

= 1000 x V₁ = 1 x 10 ml

V₁ = 10/1000 = 0.01ml

Therefore, we need 0.01ml of sample volume and 10-0.01 = 9.99 ml of diluents to make the solution of 10ml into 1:1000 dilutions.