Predict the effect substantial changes in temperature pH or

Predict the effect substantial changes in temperature, pH or salt concentration on protein structure and function.

Solution

Effect of Tempreture on the strucutre of Protein

high tempreture can disturb the secondry structure of a protein by disturbing hydrogen bond, and it can also disturb the hydrophobic interaction. Heat increases the kinetic energy and causes the molecule to vibrate so rapidly and its leads to disruption of hydrogen bond and hydrophobic interaction.

Effect of PH on the strucutre of Protein

Changes in PH result in protonation and deprotonation of the side group of amino acid of protein which alter hydrogen bonding and salt bridge pattern.

Effect of Salt on the strucutre of Protein

When salts added to the system , water solvates the salt ions and as salt concentration increases water is removed from around the protein, eventually exposing the hydrophobic group. hydrophobic groups onthe protein molecule can interact with thode of other, resulting in aggregation and thus precipitation.