You have cloned a gene that encodes a musclespecific enzyme

You have cloned a gene that encodes a muscle-specific enzyme. The transcription startsite is noted with an arrow and the blue colored region represents the transcript. start codon stop codon Red-introns 5\'

Solution

The regulatory regions that impact transcription lies upstream to the start codon of the transcript. So, the regulatory genes might appear at location 1 and 5.

An untranslated region (or UTR) is found on two strands of the DNA, one on each side of a coding sequence on a strand of mRNA. If it is found on the 5\' side, it is called the 5\' UTR.

In the above given sequence, location 4 contains 5’ UTR.