Homework SourseSolution Lysis time min Urea 60 MW immediately Glycerine 92
Solution
Lysis time (min)
Urea (60 MW)
immediately
Glycerine (92 MW)
Didn’t lyse after 30 min
Glucose (180 MW)
Didn’t lyse after 30 min
Sucrose (342 MW)
Didn’t lyse after 30 min
Each of the solutions was 0.5 M. Convert this to osmolarity – show all work.
| Solution | Lysis time (min) |
| Urea (60 MW) | immediately |
| Glycerine (92 MW) | Didn’t lyse after 30 min |
| Glucose (180 MW) | Didn’t lyse after 30 min |
| Sucrose (342 MW) | Didn’t lyse after 30 min |
Solution
Solution
Molarity
Osmolarity
Urea (60 MW)
0.5
0.5
Glycerine (92 MW)
0.5
0.5
Glucose (180 MW)
0.5
0.5
Sucrose (342 MW)
0.5
0.5
Total osmolarity
2.5
Osmolarity = molarity x n
n: number of particles that dissociated from the solute molecule.
For non ionizable solutes = n=1
| Solution | Molarity | Osmolarity |
| Urea (60 MW) | 0.5 | 0.5 |
| Glycerine (92 MW) | 0.5 | 0.5 |
| Glucose (180 MW) | 0.5 | 0.5 |
| Sucrose (342 MW) | 0.5 | 0.5 |
| Total osmolarity | 2.5 |
