Please go through this article and make a very detailed list

Please go through this article and make a very detailed list of all techniques used by the researchers and what the technique was used to discover or investigate. Be as specific as possible.

article: https://www.dropbox.com/s/i9fj6fju1c5yhoa/Erez_etal_ViralCommunication_Nautre-2017.pdf?dl=0

Solution

Techniques used by the researchers are as follows:

1. Media Preparation- two types of medium prepared i.e. A. Conditioned Media from culture of Bacillus subtilis strain 168 infected with four different phages- phi20, phi105, rho14 & phi3T and, B. Control Media.

2. Incubation- conditioned media was infected with bacteria growing at mid log phase and then this conditioned media was incubated for 3 hours.

3. Filtrartion- this conditioned media after incubation was finely filtered to collect small molecules.

4. Sequencing of the Total DNA of bacteria.

5. Mass Spectrometry- for the confirmation of presence of SAIGRA peptide in conditioned medium, but not in the control medium.

6. Semi-quantitative PCR assay- for examination of phage phi3T integration into the B.subtitils genome during infection.

7.RNA Seq- to RNA extracted from bacteria during infection with & without peptide & change in the expression was observed for for aimX which was immediately downstream to the AimR DNA-binding site.

8. ChIp-Seq Assay- after phage infection with & without presence of artibrium peptide. Cell cultures of B.subtilis 168 & AimR-His tag complementation strain.

9. AimR Purification- presence of protein migrates as homodimer in gel filteration column.

10.Microscaled thermophoresis- Two-step purified 6xHis-tagged AimR stored in Tris-NACL buffer at -80⁰ C was thawed on ice & centrifuged before analysis.

11.SDS-PAGE Gel- for the purity verification by protein running as a single band.