ConNgation and Gene Mapping in Baderia Congugation and Gene

ConNgation and Gene Mapping in Baderia Congugation and Gene Mapping in Bacleria Each mutant minimalmedum only if it sappiemested with a specifs In your resersch an animo acid biosythesis E ook, you have dertted three auprotrophic mutants in a mutants requre gyoine. Phet mutants reque phenylalarina As part your newesech you decide to mmp the loootions of the three mutml kari Part A Genotyping exconjugants on supplemented media To muke rough map of thw ys2 gha and phef you perform interrupted ooruption experment between the folowing two Amph inseates sensitivity to ampieilin AmpR indicates resistance to ampeln You intempt ooriuntion after 1 min. 2 mn, and 3 min and plate exconjugants or minimal medium containing ampiciln und vunous eombanators of lysine, gyeine, und pherylalarie. You then scow the resulting plates for the presence of Gokries Based on the results shown below, what are the possible genotypes of the colonies that grew on each medium? Anower the question by dragging one pink label to each pink target one white label to each white taraet and one blue label to each blue target Labels may be used not at all. Medium Time of interruption includes ampiciliin colonies Colonies Minimal medium lysine glycine Minimal medium phenylalanine glyd co colonies colonies Minimal medium phenylalanine lysine enonies colonies colonies 3:02 PM Ask me anything 3/25/2017 F6 Prt\'Scr nsert.

Solution

Amp^R Phe^1-, gly^3-,lys^2- is the possible genotype of the colony that grows on the media as first Amp^R gene entered as it is required to grow on the media containing ampicillin.After 2 minutes with phenylalanine growth was there but after three minutes growth was observed with both lysine and glycine.