The following diagram shows a Southern blot of restriction d

The following diagram shows a Southern blot of restriction digested genomic DNA from an elf Mother (M) and elf Father (F) and four potential elf children (C1 to C4) probed with a VNTR DNA sequence. The restriction enzyme used was Not I. Another elf (F2) is claiming to be the father of child C4. Assume the mother, M, is the real mother of these four children.

a) Which children are the biological children of the elf Father (F)?

b) Which children if any, could be elf F2\'s children?

c) Consider the data in the lane labelled “markers”. According to this lane only, what is the minimum number of loci which are being examined?

d) If the number of loci indicated in the previous question represents the total number of loci being examined, the profile of C4 must include a minimum of how many heterozygous loci?

PLEASE ANSWER ALL QUESTIONS AND EXPLAIN I WANT TO UNDERSTAND!

M F C1 C2 C3 C4 F2 Markers | aJi lllllllll lllll 8]ll ll lll llll l li ll C3 0-------------- lll lllllll llll I llll Ill I l Ill I lll llllllll -llll I lll lll l li l f-i k

Solution

a). C4 and C2 are the biological childrens of elf Father

b). C3 is the child of FATHER 2

c) Minimum 13 loci are being examined becaus marker is given for 25 loci but the minimum no. of loci in the test samples ( for example. M) is 13.

d) 4 hetrozygous loci because 4 loci are matching with both mother and father

c)