Identify the following point mutations in DNA and in RNA as

Identify the following point mutations in DNA and in RNA as a 1) transition, 2) transversion, or 3) reading frameshift. Answer using 1, 2 or 3. a) A to G ________ b) C to T _________ d) C to G __________ e) T to A ____________ c) UUG CUA, AUA to UUG CUG AUA 2a. If the coding segment of an mRNA with the sequence 5\' AUGUUUCCCAAAGGG 3\' is translated, what amino acid sequence will be produced? 2b. What would be the coding sequence on the template DNA? 2c. What would be the coding sequence on the non-template strand of DNA?

Solution

1 a). 1 (Transition) = Purine transitioned into Purine

1 b). 1 (Transition) = Pyrimidine transitioned into Pyrimidine  

1 c). 1 (Transition) =  Purine transitioned into Purine

1 d). 2 (Transversion) = Pyrimidine transversioned into Purine

1 e). 2 (Transversion) = Pyrimidine transversioned into Purine

2 a). 5¹-Met-Phe-Pro-Lys-Gly-3¹ = Amino acid sequence

2 b). 3¹-TACAAAGGGTTTCCC-5¹ = Coding sequence on the template DNA

2 C). 5¹-ATGTTTCCCAAAGGG-3¹ = Coding sequence on the non-template DNA