2 Name the first restriction enzyme derived from Providencia

2. Name the first restriction enzyme derived from Providencia stuartii, a Gram negative bacterium that is commonly found in soil, water, and sewage

3.

Consider these descriptions of different enzymes: Enzyme A: cuts amino acid bonds in protein molecules; degrades protein molecules into small peptides Enzyme B: cleaves double-stranded DNA at specific sequence; can cut circular DNA that has no free ends works with single and double-stranded DNA: cannot process circular DNA b/c it does linear DNA molecules; Enzyme C: chips off single nucleotides from free ends of not expose free ends To which category do restriction enzymes belong Enzyme A Enzyme B Enzyme C D. Nothing above

Solution

QUESTION 1 - Enzyme B

Reason - A restriction enzyme (or restriction endonucleases) recognizes a specific base pair sequence in DNA called a restriction site and cleaves the DNA, within the sequence.

QUESTION 2 - Pstl restriction enzyme .

Question 3 - DNA #3 Are BLUNT ENDS ,

REASON - In molecular biology, a blunt end may result from the breaking of double-stranded DNA; however, there are no overhangs or unpaired bases.