a The concentration of the plasmid used in this experiment w

a) The concentration of the plasmid used in this experiment was 0.002 ug/ul. So how much plasmid DNA did your group use? Include your calculation! We add 10ul of the pGLO solution to the +pGLO tube and add 10 ul of water to the -pGLO tube.

b) Assume you count 250 colonies on the +pGLO LB/amp/ara plate and a confluent lawn of bacteria on the +pGLO LB/amp plate. Did you expect this result? If not, give a detailed explanation! How could you prove your theory?

Solution

a. The plasmid concentration is 0.002 ug/ul or 2 ng/ul. If 10uL of plasmid is taken in both the tubes which makes it 20 ng of plasmid DNA in both +pGLO and -pGLO tubes.

b. The ara in the first plate is acting like a selection marker which makes the colonies which have this gene are able to survive.